1. Don't be gender specific when you reserve your room. Always use your first initial and your last name.
2. Avoid rooms that are in isolated areas of the building. Asked to be placed in more heavily trafficked areas.
3. Request that your room number and location be kept private. A good desk clerk will write your room number down rather than say it out loud. Others within hearing distance could use this information to gain access to your room.
4. Don't accept ground-floor rooms that have easy access from outside such as a balcony or fire escape.
5. Make sure all the doors and windows of your room can be locked from the inside and can't be opened from the outside with a key.
6. Carry a rubber door stopper that can be easily installed on an inward-opening door.
7. Never give your room number to anybody. If someone is coming to meet you arrange for a meeting in the lobby.
8. Instead of filling out a room service card and leaving it on the outside of your door, call room service directly. There is no need to alert potential wrongdoers that you are the sole person in the room.
9. Ask that your room service order be delivered by a woman.
10. Just because someone knocks on your door and identifies themselves as a hotel employee is no reason to open the door. If you are the least bit suspicious ask for their name and why they are there. Call the front desk to verify.
11. Avoid solitary situations. If your hotel has underground parking ask that a hotel employee park your car for you.
12. Finally, There is no harm in relaxing in the hotel bar but never leave your drink unattended. Social deviants find this a perfect time to drop date rape drugs into your beverage and leave you vulnerable to their intentions. The good news is that now there is a product to test your own drink and foil these wrong-doers. See: http://www.drinksafetech.com